电刺激对过氧化氢诱导衰老肌管细胞能量代谢的影响

Effect of Electrical Stimulation on Energy Metabolism of Senescent Myoduct Cells Induced by Hydrogen Peroxide

投稿时间：2023-06-06

DOI：10.12064/ssr.2023060601

英文关键词:electrical stimulation C₂C₁₂ muscle tube H₂O₂ energy metabolism

基金项目:辽宁省教育厅项目（L2014430）；校高端科研成果培育资助项目（203070142207）

作者	单位
苏艳红	辽宁师范大学体育学院，辽宁大连 116029
毕亮	辽宁师范大学体育学院，辽宁大连 116029
龚文翰	辽宁师范大学体育学院，辽宁大连 116029
宋涛	遵义医科大学基础医学院，贵州遵义 563000
李婷婷	辽宁师范大学体育学院，辽宁大连 116029
张世超	北京体育大学，北京 100084

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中文摘要:

目的：运用电刺激肌管细胞收缩模拟骨骼肌收缩，并观察其对过氧化氢（H₂O₂）诱导的C₂C₁₂细胞衰老模型能量代谢相关指标表达的影响，以此研究体外肌管收缩对衰老骨骼肌细胞能量代谢影响的分子机制。方法：使用不同浓度的H₂O₂诱导肌管细胞建立衰老模型，并用β-半乳糖苷酶染色检测衰老水平；电刺激干预培育成功的衰老肌细胞，并分为对照组（C组）、H₂O₂诱导衰老组（H组）、对照+电刺激组（CE组）、H₂O₂诱导衰老+电刺激组（HE组）。各组细胞通过Western blot法对PGC-1α蛋白表达进行检测，ATP水平测试盒测量ATP水平。结果：经过浓度为100 μmol/L H₂O₂诱导肌管细胞24 h后成功构建骨骼肌细胞衰老模型。与C组比较，H组的ATP水平表达呈非常显著性的下降（P＜0.01）；CE组的ATP水平较C组呈非常显著性升高（P＜0.01）；HE组的ATP水平虽低于C组，但高于H组，且呈非常显著性差异（P＜0.01）；与C组相比，H组PGC-1α蛋白表达下降，呈非常显著性差异（P＜0.01）；HE组PGC-1α蛋白表达虽低于C组，但较H组呈非常显著性升高（P＜0.01）。结论：H₂O₂是诱导骨骼肌肌管细胞衰老的一种有效方式，衰老肌管细胞的ATP和PGC-1α蛋白表达水平显著下降，进行电刺激干预后可以有效提高衰老肌管细胞ATP水平和PGC-1α蛋白表达。

英文摘要:

Objective: Muscle tube cell contraction was simulated by electrical stimulation, and its effect on the expression of energy metabolism related indicators in C₂C₁₂ cell aging model induced by hydrogen peroxide (H₂O₂) was observed, so as to study the molecular mechanism of the effect of muscle tube contraction on energy metabolism of aging skeletal muscle cells in vitro. Methods: The senescence model was established by inducing myoduct cells with different concentrations of H₂O₂, and the senescence level was detected by SA-β-galactosidase staining. Electrical stimulation interfered with the successful growth of senescent muscle cells which were divided into control group (group C), H₂O₂-induced group (group H), control + electrical stimulation group (group CE), and H₂O₂-induced + electrical stimulation group (group HE). For cells in each group, PGC-1α protein expression was measured by Western blot and ATP level was measured by ATP level test kit. Results: Myotube cells were treated with 100 μmol/L H₂O₂ for 24 hours, and the aging model of skeletal muscle cells was successfully established. Compared with group C, ATP level in group H was significantly decreased(P＜0.01); The ATP level in CE group was significantly higher than that in C group(P＜0.01). The ATP level in HE group was lower than that in C group, but higher than that in H group, and the difference was very significant(P＜0.01). Compared with group C, the expression of PGC-1α in group H was significantly decreased(P＜0.01). Although the expression of PGC-1α in HE group was lower than that in C group, it was significantly higher than that in H group(P＜0.01). Conclusion: H₂O₂ is an effective way to induce senescence of skeletal muscle myoduct cells. The protein expression levels of ATP and PGC-1α in senescent C₂C₁₂ cells decreased significantly. After electrical stimulation intervention, ATP level and PGC-1α protein expression in senescent C₂C₁₂ cells could be effectively increased.

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